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KMID : 0545120180280030473
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Journal of Microbiology and Biotechnology
2018 Volume.28 No. 3 p.473 ~ p.481
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Exploring the Metabolomic Responses of Bacillus licheniformis to Temperature Stress by Gas Chromatography/Mass Spectrometry
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Dong Zixing
Chen Xiaoling
Cai Ke
Chen Zhixin
Wang Hongbin
Jin Peng
Liu Xiaoguang
Permaul Kugenthiren
Singh Suren
Wang Zhengxiang
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Abstract
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Owing to its high protein secretion capacity, simple nutritional requirements, and GRAS (generally regarded as safe) status, Bacillus licheniformis is widely used as a host for the industrial production of enzymes, antibiotics, and peptides. However, as compared with its close relative Bacillus subtilis, little is known about the physiology and stress responses of B. licheniformis. To explore its temperature-stress metabolome, B. licheniformis strains ATCC 14580 and B186, with respective optimal growth temperatures of 42oC and 50oC, were cultured at 42oC, 50oC, and 60oC and their corresponding metabolic profiles were determined by gas chromatography/mass spectrometry and multivariate statistical analyses. It was found that with increased growth temperatures, the two B. licheniformis strains displayed elevated cellular levels of proline, glutamate, lysine, pentadecanoic acid, hexadecanoic acid, heptadecanoic acid, and octadecanoic acid, and decreased levels of glutamine and octadecenoic acid. Regulation of amino acid and fatty acid metabolism is likely to be associated with the evolution of protective biochemical mechanisms of B. licheniformis. Our results will help to optimize the industrial use of B. licheniformis and other important Bacillus species.
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KEYWORD
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Bacillus licheniformis, gas chromatography/mass spectrometry, temperature-stress metabolome, amino acid and fatty acid metabolisms
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